Box 1: Yes -
NVA1 with IP (NVA-network virtual appliance) 192.168.0.4 is on the DMZ subnet. It will use route 10.0.0.0/16 to the on-premises network.
Box 2: No -
VM2 has IP address 192.168.2.4 and is on the BackEnd subnet. VM2 will not use the RT1 route table, and will not reach the on-premises network through NVA1.
Box 3: Yes -
VM1 with IP address 192.168.1.4 is on the FrontEnd subnet, and will use the RT1 routing table. It will use Route2 and Next Hop IP address 192.168.0.4, IP address of NVA1, to reach VM2.